Table 2.
Phenotype of lean and DIO Gal-1 KO mice
| WT Lean | Gal-1 KO Lean | WT DIO | Gal-1 KO DIO | |
|---|---|---|---|---|
| BW (g) | 28.0 +/− 1.0 | 27.6 +/− 0.1 | 46.3 +/− 1.0*** | 45.0 +/− 1.1*** |
| % fat mass | 8.2 +/− 2.4 | 13.1 +/− 2.5 | 40.9 +/− 2.3*** | 53.4 +/− 3.9*** oo |
| VAT adipocyte size (μm) | 136.7+/− 21.2 | 115.3 +/− 8.4 | 347.3+/− 10.7*** | 374.9 +/− 17.6*** |
| SAT adipocyte size (μm) | 93.5 +/− 18.7 | 93.6 +/− 26.5 | 244.6 +/− 33.7*** | 274.8 +/− 31.7*** |
| Leptin (ng/ml) | 1.75 +/− 0.28 | 0.96 +/− 0.52 | 57.1 +/− 20.47*** | 33.90 +/− 2.08*** |
| Adiponectin (μg/ml) | 4.91 +/− 0.42 | 5.18 +/− 0.19 | 4.65 +/− 0.56 | 4.74 +/− 0.46 |
| Glucose (mg/dl) | 159.2 +/− 16.3 | 157.6 +/− 12.2 | 229.6 +/− 13.4** | 201.2 +/− 11.0** |
| Insulin (ng/ml) | 1.06 +/− 0.21 | 0.74 +/− 0.08 | 1.76 +/− 0.15* | 2.49 +/− 0.51*** |
WT and Gal-1 KO mice were fed regular chow (Lean) or HFD (DIO) for 13 weeks. Blood was obtained for measurement of leptin, adiponectin, glucose and insulin. Fat mass was evaluated by DXA. Mean size of adipocytes in VAT and SAT was evaluated using ImageJ. Data are mean +/− SEM of 5 mice per group.
*
p<0.05,
**
p<0.05,
***
p< 0.001 versus respective lean;
oo
p< 0.01 versus WT DIO.