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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Nutr Biochem. 2012 Dec 15;24(6):1146–1152. doi: 10.1016/j.jnutbio.2012.08.015

Figure 1. γ-TE inhibited LPS-stimulated IL-6 but had no effect on TNFα, IL-10 or COX-2 in RAW267.4 macrophages.

Figure 1

After preincubated with tocopherols (Panel A) and γ-TE (Panel B, C, D and E) for 14–16 h, RAW 264.7 cells were stimulated by LPS (0.1μg/ml) for 16–18 h. Media were collected to measure IL-6, TNFα and IL-10 and cells were collected to analyze COX-2 expression using immunoblot. αT50, γT10, γT25, γT50, δT10, δT25, δT50, γTE5, γTE10 and γTE20 stand for the corresponding vitamin E at the indicated concentrations (μM), respectively. Relative cytokines are the ratio of cytokine secreted by cells treated with tested compounds to that of solvent controls in the presence of LPS. Ctrl is non-LPS control. Data are shown as mean±S.D. *p<0.05 indicates significant difference between vitamin E-treated and solvent control cells (n >2 per bar).