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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Nutr Biochem. 2012 Dec 15;24(6):1146–1152. doi: 10.1016/j.jnutbio.2012.08.015

Figure 5. Knockdown of C/EBP-β diminished IL-6 and γ-TE inhibited LPS-induced C/EBPβ and G-CSF.

Figure 5

Panels A and B: RAW cells were transfected with C/EBPβ siRNA and then stimulated with LPS for 8 h. Whole protein was probed for C/EBPβ by Western blot. The relative IL-6 was the ratio of IL-6 secreted by cells transfected with C/EBPβ siRNA to those with control siRNA (ctrl-siRNA) in the presence of LPS. Ctrl is no-LPS control. Panel C: RAW cells were pretreated with γ-TE at indicated concentrations (μM) for 14 h and then stimulated with LPS for 16 h. Total proteins were immunoblotted with anti-C/EBPβ, C/EBPδ or β-actin. Panel D: G-CSF was measured in the media which were used to detect IL-6 in Figure 1. Data are shown as mean±S.D. *P<0.05 and **P < 0.01 were the difference between treated and control cells. n >2 per bar.