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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Obesity (Silver Spring). 2013 Feb;21(2):297–305. doi: 10.1002/oby.20002

Figure 6. GLRX1 is elevated in visceral adipose of obese rats.

Figure 6

Peri-renal adipose, liver and gastrocnemius (Gastroc) muscle were isolated from rats fed a 10% fat calorie diet or 45% fat calorie diet and analyzed for GLRX1 protein content by western blot or total GLRX activity. Western blot and blot quantitations are shown for adipose (A) and liver (B). GLRX activity for liver and skeletal muscle are presented in (C). Note: GLRX activity in peri-renal adipose was below the limit of detection with the method used. Data are the mean ± S.D.; n = 5. Data 10% fat calorie and 45% fat calorie groups were compared with student’s t-test. * p < 0.05. 10μg of protein was loaded per lane for western blots. Recombinant GLRX1 (1 ng) was used for the adipocyte blot and 0.5 ng of GLRX1 for the liver blot.