Figure 3. Network Neighborhood Contains Clock Modulating Components.

Systematic RNAi-mediated downregulation of network neighborhood genes in dexamethasone-synchronized U2OS cells harboring a Bmal1-promoter luciferase reporter. Shown are altered oscillation dynamics (red dots with corresponding fit lines) for 16 genes achieved by individual RNAi constructs (see Table S2). For twelve genes, two RNAi constructs resulted in similar phenotypes, for nine genes only one construct was available in our laboratory library. Black dots with corresponding fit lines are controls representing the mean values of at least 80 irrelevant constructs. Period deviations from controls are shown.