Table 2.
Sequences of primers used for Real-Time PCR
| PCR Primers | Sequence of Forward (F) and Reverse (R) Primers | Reference |
|---|---|---|
| GAPDH | F-5′-gAACATCATCCCTgCATCCA-3′ R-5′-CCAgTgAgCTTCCCgTTCA-3′ |
(29) |
| RBP4 | F-5′-gACAAggCTCgTTTCTCTgg-3′ R-5′-AAAggAggCTACACCC-3′ |
(30) |
| RARα | F-5′-AggCCATCACAACTACCTgC-3′ R-5′-ggAAAgAAgAAggCgTAggg-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| RARβ | F-5′-CAgCTgggTAAATACACCACgAA-3′ R-5′-ggggTATACCTggTACAAATTCTgA-3′ |
(31) |
| RARγ | F-5′-TgCCAgTACTgCAggCTAC-3′ R-5′-TCTgCACTggAgTTCgTggTgTACT-3′ |
(32) |
| RXRα | F-5′-CTTTgACAgggTgCTAACAgAgC-3′ R-5′-ACgCTTCTAgTgACgCATACACC-3′ |
(33) |
| RXRβ | F-5′-CCTCTggACgATCAggTCAT-3′ R-5′-TgTCACgATTTTggACACT-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| RXRγ | F-5′-AggCAggTTTgCCAAgCTTCTg-3′ R-5′-ggAgTgTCTCCAATgAgCTTgA-3′ |
(34) |
| Acrv1 | F-5′-TCCgTggAgAAggAgTATgC-3′ R-5′-CACgggCATCTAgACCTTgT-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| c-kit | F-5′-TCCCAgAAACAggCTgAgTT-3′ R-5′-TTCATgTgATTgCCCAggTA-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| Ccna1 | F-5′-CAgCTATTCTCCTggCTTCg-3′ R-5′-CTgATgCACACTCCTTgACg-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| Cdc25c | F-5′-CCCTCggTgAAgACTCTgAA-3′ R-5′-CCgAgTCgTggAgTTTgTCT-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| Oct4 | F-5′-ACCATgTTTCTgAAgTgCCC-3′ R-5′-TgggAAAggTgTCCCTgTAg-3′ |
Primer-BLAST Primer designing tool (NCBI) |
| Stra8 | F-5′-CgATCTCTCCCACTCCTCCT-3′ R-5′-ggAgTCTggCCTTTTTCTCC-3′ |
Primer-BLAST Primer designing tool (NCBI) |