Figure 7. Analysis of mast cell (MC) phenotype in NP, PCa and CP.

(A) Human pancreatic tissue samples from NP, PCa and CP were double-immunolabeled for perineural MC-tryptase (red) and MC-chymase (green). In all three entities, the vast majority of perineural MC demonstrated double immunoreactivity (yellow in overlay) for MC-tryptase and -chymase. In CP, there were significantly greater relative amounts of double-immunoreactive MC among patients with pain than among those without pain. The white scale bars indicate 100 µm. (B) In accordance with increased perineural MC-tryptase in painful PCa and CP, the perineural MC chymase immunoreactivity was significantly greater among PCa and CP patients with pain than in patients without pain. Results are expressed as median (Minimum; Maximum).