Figure 1. Agonist-induced total IP accumulation in HEK293 cells transiently expressing equal amounts of the WT (A) or E129V mutant (B) of human TP receptor.
Total IP accumulation was measured after incubation in the absence (basal) or presence of increasing concentrations of the indicated agonists for 30 min. Data are expressed as dpm/well. Error bars represent mean±SE of at least three independent experiments each performed in duplicates or triplicates (For the sake of clarity, in panel B, error bar direction of U46619 and I-BOP data is above and below, respectively). Curves are computer generated from the simultaneous analysis of at least three independent experiments. Values for EC50 and significant differences from WT are shown in Table 2.