Figure 4. Functional analysis of basal and agonist induced total IP accumulation in HEK293 cells transiently expressing the double mutant E129V/E240V of human TP receptor without and following rescue with 1.

µM SQ29,548 for 18 h. A. Total IP accumulation in basal condition and following 30 min stimulation with 1 µM U46619. B. Concentration-response curves of U46619. Error bars represent mean±SE of at least two independent experiments each performed in duplicates.