. 2013 Mar 28;8(3):e60475. doi: 10.1371/journal.pone.0060475

Table 3. Affinities of the agonists for the binding site of the receptor labelled by [³H]SQ29,548 in HEK293 cells transiently expressing the WT or the mutant human TP receptors.

Agonist	WT K_i, nM±%CV	E129V K_i, nM±%CV	Affinity ratio^a
U46619	169.90±13	16.32±18^**	10.4
I-BOP	24.27±18	3.90±21^**	6.2
8-isoPGE₂	2916±15	547.6±2^**	5.3
8-isoPGF_2α	12930±19	2908±11^**	4.4

Ki values were obtained by simultaneous analysis of at least 3 independent competition experiments analyzed with GraphPad Prism implemented with the LIGAND model (see Data and statistical analysis).

Affinity ratio was calculated as the ratio of the K_i for the WT TP receptor over the K_i of the E129V mutant.

^**

p<0.01 vs. WT.

Table 3. Affinities of the agonists for the binding site of the receptor labelled by [3H]SQ29,548 in HEK293 cells transiently expressing the WT or the mutant human TP receptors.

Table 3. Affinities of the agonists for the binding site of the receptor labelled by [³H]SQ29,548 in HEK293 cells transiently expressing the WT or the mutant human TP receptors.