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. 2013 Mar 28;8(3):e60074. doi: 10.1371/journal.pone.0060074

Figure 2. Effect of silybin and its derivatives on apoptosis in HTB9 cells.

Figure 2

(A–B) HTB9 cells were treated with 30 µM dose of silybin (SB), 2,3-dehydrosilybin (DHS), 7-O-methylsilybin (7OM), and 7-O-galloylsilybin (7OG). After 24 or 48 h of each treatment, cells were collected and apoptotic population was determined by Hoechst assay as detailed in the ‘Materials and Methods’. In the bar diagrams, each data-point is representative of mean ± SD of 3 samples. (A–B, western blot insert) Cells were also collected following silybin or its derivatives treatment and analyzed for cPARP protein level by Western blotting. In each case, membranes were also stripped and re-probed with α-tubulin antibody to check protein loading. *p≤0.001.