Figure 3. Effect of low doses of pure optical isomers of silybin and its derivatives on HTB9 cell growth.

HTB9 cells were treated with 5 and 10 µM doses of silybin A, 2,3-dehydrosilybin A, 7-O-methylsilybin A, 7-O-galloylsilybin A or silybin B, 2,3-dehydrosilybin B, 7-O-methylsilybin B, 7-O-galloylsilybin B. After 24 or 48 h of each treatment, total cell number was determined as detailed in the ‘Materials and Methods’. In the bar diagrams, each data-point is representative of mean ± SD of 3 samples. *p≤0.001; #p≤0.01; $p≤0.05.