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. 2013 Mar 28;9(3):e1003400. doi: 10.1371/journal.pgen.1003400

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© 2013 Esnault et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of the simian placenta, with an enlarged villus bathed in maternal blood and displaying –from the maternal to the fetal side- the syncytiotrophoblast (ST) layer, the mononucleated cytotrophoblasts (CT) and the fetal vessels. (B) Serial sections of macaque (upper row) and human (two lower rows) placenta. (columns 1–3) in situ hybridization with digoxigenin-labeled envV2 sense (negative control, column 1) and antisense (columns 2,3) riboprobes, revealed with an alkaline phosphatase-conjugated anti-digoxigenin antibody. (column 4) immunohistochemical staining of the Ki67 nuclear antigen. (columns 3,4) enlarged views with the empty arrowheads pointing to the syncytiotrophoblast positively stained for envV2 mRNA, and the filled arrows to Ki67-marked mononucleated cytotrophoblasts.