Skip to main content
. 2013 Mar 28;8(3):e59866. doi: 10.1371/journal.pone.0059866

Figure 6. Smc5/6 genes are not required for G2/M and S phase checkpoints induced by genotoxic agents.

Figure 6

(A) Wandering third instar larvae were irradiated with 40 Gy of ionizing radiation and the eye-antenna discs were dissected and fixed 15 minutes, 30 minutes, 1 hour or two hours after radiation, with discs from unirradiated larvae serving as controls. Representative images of PH3 staining for mitotic cells in eye-antenna discs from control (WT, FRT82B) and Smc6, (jnjR1/jnjX1) transheterozygous larvae are shown. (B) Eye-antenna discs from wandering third instar larvae were incubated with or without HU before adding BrdU to the incubation solution. Representative images of BrdU staining for cells in S phase in eye-antenna discs from control (WT, FRT82B), transheterozygous Smc6 (jnjR1/jnjX1) or transheterozygous MAGE (sstRZ/sstXL) eye-antenna discs are shown.