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. 2013 Mar 28;8(3):e59866. doi: 10.1371/journal.pone.0059866

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© 2013 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative eye phenotypes of MAGE (EGUF/+; FRT82B sst^RZ/FRT82B GMR-hid, loss of MAGE in eye cells), ey>ATMi (knockdown of ATM in eye cells), ey>ATMi;MAGE (EGUF/UAS-ATM-RNAi;FRT82B sst^RZ/FRT82B GMR-hid, loss of MAGE and knockdown of ATM in eye cells) and ey>ATRi;MAGE (EGUF/UAS-ATR-RNAi;FRT82B sst^RZ/FRT82B GMR-hid, loss of MAGE and knockdown of ATR in eye cells) flies that were reared on either standard media or media containing 2 mM caffeine. The EGUF system carrying the eyeless-Gal4 driver was used to drive the UAS-RNAi transgenes in the eye and also makes the eye homozygous for MAGE (sst^RZ). Controls for the effects of each eyeless-driven RNAi alone were carried out for ATM and ATR resulting in wild type appearing eyes, but only the results of ATM RNAi are shown here as an example. (B) Representative eye phenotypes of MAGE knockdown (eyeless-Gal4/+;UAS-MAGE-RNAi/UAS-Dicer2, knockdown of MAGE in eye cells) and MAGE Rad51 double knockdown (eyeless-Gal4/UAS-SpnA-RNAi;UAS-MAGE-RNAi/UAS-Dicer2, knockdown of MAGE and Rad51 in eye cells) flies that were reared on either standard media or media containing 2 mM caffeine.