Table 1. Comparison of microtubule dynamic instability measured by EGFP-tubulin or 2G4-GFP expression in LLCPK cells.

Dynamic Instability Parameters	2G4-GFP	EGFP-Tub	EGFP-Tub [20]	EGFP-Tub [18]
Growth Rate (µm/min)	8.5±4.8	10.0±5.0	11.5±7.4	8.5±5.8
Shortening Rate (µm/min)	12.0±9.4	16.9±9.9	13.1±8.4	11.3±7.9
Catastrophe Frequency (s−1)	.027±.004	.05±.01	.026±.024	.053±.003
Rescue Frequency (s−1)	.086±0.13	.106±.024	.175±.104	.086±.005
Percent Time in growth, shorteningand pause	63.7, 19.3, 27	54.2, 26.6, 19.2	15, 11.5, 73.5	40.4, 26, 36.5
Dynamicity (µm/min)	8.2±5.6	9.34±5.5	4±3.5	5.3±2.7

Parameters of dynamic instability measured using EGFP-α-tubulin (EGFP-Tub) as a tracer, expressed at about 10% of total a-tubulin levels, compared to that measured using 2G4-GFP bound to the external surface of microtubules. Published data sets derived from LLCPK cells expressing EGFP-α-tubulin are also included for comparison. Cells measured in reference [18] also expressed CFP.