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. 2013 Mar 28;8(3):e60612. doi: 10.1371/journal.pone.0060612

Figure 5. Infection of macrophages with R. equi affects host cell size and morphology.

Figure 5

Macrophage monolayers were infected with ATTO 488 labeled virulent R. equi 103+ or attenuated R. equi 103− strains at increasing multiplicities (MOI) of infection. Monolayers were fixed and labelled 24 h post-infection. The cell size (panel A) and cell roundness (panel B) of infected cells were compared to control cells (not infected, indicated as MOI = 0). Green bars: no intracellular bacteria; blue bars: 1 to 5 intracellular bacteria; red bars: more than 5 intracellular bacteria. +: R. equi 103+; −: R. equi 103−. Error bars denote the standard deviation of the mean.