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. 2013 Mar 28;8(3):e60384. doi: 10.1371/journal.pone.0060384

Figure 8. Rep1I202T does not associate with the plasmid STB locus but retains interaction with SUMO.

Figure 8

(A) Association of wild type and I202T mutant Rep1 (Rep1I202T) with STB in cir0 yeast was monitored using a one-hybrid assay as described in the legend to Figure 5. (B) Interaction of Rep1 and Rep1I202T with SUMO and with Rep2 was monitored in cir0 yeast using a two-hybrid assay as described in the legend to Figure 1. Levels of the Rep1 and Rep1I202T fusion proteins in the (C) one-hybrid and (D) two-hybrid reporter yeast strains, in A and B respectively, were monitored by western blotting analysis with antibodies specific for Pgk1 (C and D, bottom), anti-HA (C, top) and anti-LexA (D, top).