Figure 5. Comparison of the transmembrane proteins identified from rat liver PM-enriched fraction in three different detergent-assisted methods.

(A) Comparison of the distributions of all the identified integral membrane proteins as a function of TMDs. The data merged from triplicate analysis were used for comparison. (B) Comparison of sequence coverages of the integral membrane protein isoform GLAST-1 of excitatory amino acid transporter 1 (IPI00324377) identified by RGS, SDC or SL method. The sequence coverages were visualized with the TOPO2 transmembrane protein graphics program. The tryptic peptides identified by all three methods were in red, and those newly identified only by SL method were in blue, whose two tryptic cleavage sites inside the TMDs are indicated with black arrows.