FIGURE 4.

VPS26 is required for VPS29 endosomal recruitment. Immunoblot analysis of total, cytosolic, and microsomal proteins from wild-type (WT) and vps26a-1 vps26b-1 plants using anti-VPS29, anti-H⁺-ATPase, and anti-cytosolic FBPase antibodies. H⁺-ATPase and FBPase were used as markers of membrane and cytosolic fractions, respectively. The arrow corresponds to VPS29, and the asterisk indicates a protein that is nonspecifically recognized by the anti-VPS29 antibody in microsomal fraction because it is detected in vps29-3 mutant (top panel). Molecular mass markers are indicated on the left in kDa.