FIGURE 2.

Effects of RS and RRM domains on nuclear/cytoplasmic localization of Tra2β in SH-SY5Y cells. A, illustrations of the DNA construct to express the recombinant fusion proteins. The GFP reporter gene was fused in-frame with the full-length Tra2β (GFP-Tra2β), the N-terminal RS1 domain (GFP-RS1) or the C-terminal RS2 domain (GFP-RS2); or the full-length Tra2β lacking the RS2 domain (GFP-RS1RRM), lacking the RS1 domain (GFP-RRMRS2) or lacking both RS1 and RS2 domains (GFP-dRS12). B, fluorescent signals of GFP (or GFP fusion proteins) and nuclear staining (DAPI, blue) in the cells with expression of various GFP fusion proteins. Scale bars: 20 μm. C, Western blot images of the nuclear (N) and cytoplasmic (C) fractions of the SH-SY5Y cells with expression of various GFP fusion proteins. TBP was used as the loading control of nuclear proteins, and GAPDH was used as the loading control of cytoplasmic proteins.