FIGURE 5.

Involvement of angiomotin in the signaling cascade elicited by soluble CD146 in endothelial progenitor cells. A, analysis of signal transduction proteins modulated in response to rsCD146. Proteins that were up- or down-regulated in response to rsCD146 were characterized by antibody array (see “Experimental Procedures”). Proteins were classified as a function of the induction factor (>2 or >1.5) or the inhibition factor (<0.66 or <0.5), as compared with control condition. B, induction of p-Fak, p-Jnk, p-Akt, and p-p38 in response to rsCD146. The effect or rsCD146 was analyzed by Western blot and quantified. The effect of angiomotin silencing was also tested. A representative experiment is presented for each protein, and the average of three to six experiments is given. In the inset are shown the inhibitory effects of the Fak inhibitor 14 and of the Akt inhibitor GSK 690693 on the rsCD146-induced increase in EPC migration and proliferation, respectively. *, p < 0.05; **, p < 0.01, experimental versus control (C).