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Typical sensorgrams and affinity analysis of WT and K42A MtODCase. The left and right panels represent sensorgrams and the corresponding analyses, respectively. RU stands for resonance unit. The vertical black lines in the right panels indicate the KD values. A, wild-type MtODCase is immobilized, and binding of UMP is analyzed. B, wild-type MtODCase is immobilized, and binding of 6azaUMP is analyzed. C, K42A MtODCase is immobilized, and binding of UMP is analyzed.
