TABLE 2.

Binding affinity of various ligands to MtODCase

	Binding affinity	Temperature (reference)
	m
KD values
WT-UMP	(4.2 ± 0.2) × 10−4	25 °C
WT-6azaUMP	(2.9 ± 0.2) × 10−6	25 °C
K72A-UMP	(4.1 ± 0.3) × 10−9	25 °C
K42A-UMPa	∼8 × 10−3	25 °C
Ki values
WT-UMP	(3.3 ± 0.1) × 10−4	55 °C (20)
WT-6azaUMP	(1.24 ± 0.07) × 10−5	55 °C (20)

^a Only a rough estimate is possible because of the very low affinity pushing the analytical limits of Biacore analysis.