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. 2013 Feb 7;288(13):9028–9034. doi: 10.1074/jbc.M112.432005

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Interaction of CCNH/CDK7 with CtBP2 changes its phosphorylation and dimerization. A and B, HEK293T cells were cotransfected with GFP-tagged CtBP2 and pS4.1-CCNH (A) and pS4.1-CDK7 (B) expression constructs, as indicated. Proteins were precipitated (IP) with anti-FLAG-agarose and analyzed by Western blotting (WB) using the indicated antibodies. C, 293T cells were transfected with expression constructs as indicated. Proteins were isolated with anti-GFP-agarose and analyzed by Western blotting with a FLAG antibody. D and E, 293T cells were transfected with pS4.1-CCNH (D) or pS4.1-CDK7 (E) expression constructs. 48 hours later, proteins were isolated with anti-GFP-agarose and analyzed by Western blotting with a FLAG antibody.