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. 2013 Feb 7;288(13):9028–9034. doi: 10.1074/jbc.M112.432005

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Nonphosphorylation mutants of CtBP2 interact with CCNH/CDK7. A and B, HEK 293T cells were cotransfected with FLAG-CtBP2(S164A) or FLAG-CtBP2(S428A), together with GFP-CCNH (A) or GFP-CDK7 (B) expression vectors, as indicated. Proteins were precipitated (IP) with anti-GFP-agarose and analyzed by Western blotting with FLAG antibody. C and D, mimic phosphorylation of CtBP2 disrupts its interaction with CCNH/CDK7. HEK293T cells were cotransfected with FLAG-CtBP2(S164D) or FLAG-CtBP2(S428D), together with GFP-CCNH (C) or GFP-CDK7 (D) expression vectors, as indicated. Proteins were precipitated with anti-GFP-agarose and analyzed by Western blotting with FLAG antibody.