FIGURE 6.

Effects of APP-IP-TIMP-2 on degradation of type IV collagen by Con A-stimulated HT1080#10 cells. FITC-conjugated type IV collagen was coated on 8-well glass slides as described under “Experimental Procedures.” HT1080#10 cells pretreated with Con A as described under “Experimental Procedures” were plated onto the slides coated with FITC-conjugated type IV collagen and incubated in DME/F-12 medium supplemented with bovine fetal serum in the absence (Control) or presence of APP-IP-TIMP-2 (70 nm) or TAPI-1 (10 μm) at 37 °C for 24 h. After incubation, the cells were removed, and the FITC-conjugated type IV collagen remaining on the slides was visualized as described under “Experimental Procedures.” Scale bar, 50 μm.