FIGURE 4.

DHA, 2-deoxy-d-glucose, and d-fructose transport kinetics in GLUT8-injected oocytes. Oocytes previously injected with GLUT8 mRNA were incubated for 10 min with 0–8 mm [¹⁴C]DHA (A), 0–100 mm 2-[³H]DG (B), and 0–300 mm d-[¹⁴C]fructose (C). Oocytes were then washed, and intracellular radioactivity was quantified. A best fit curve was fitted to the collected data using the nonlinear regression function Y = V_max·X/Kt + X (SigmaPlot). Data represent mean ± S.D. of 10–20 oocytes