FIGURE 2.
UHRF2 does not affect ubiquitination but induces the SUMOylation of ZNF131. A, HEK293 cells were transfected for 24 h with FLAG-ubiquitin, HA-ZNF131, or Myc-UHRF2 alone or in combination. Cells were left untreated or were treated with MG-132 (20 μm) for 9 h. The cell lysates were immunoprecipitated (IP) with the HA antibody followed by Western blot (WB) with the indicated antibodies. Actin served as a protein loading control. B, HEK293 cells were transfected for 24 h with HA-ZNF131 alone or together with Myc-UHRF2. Cells were left untreated or were treated with MG-132 (20 μm) for 9 h. The cell lysates were immunoprecipitated with the HA antibody followed by Western blot with the indicated antibodies. Mr(K), relative molecular mass.