UHRF2 promotes ZNF131 SUMOylation at lysine 567.
A, HEK293 cells were transfected for 24 h with FLAG-UHRF2, HA-ZNF131-WT, or HA-ZNF131-K567R alone or in combination. The cell extracts were immunoprecipitated (IP) with the HA antibody followed by Western blot (WB) with the indicated antibodies. Actin was used as a protein loading control. B, HEK293 cells were transfected for 24 h with HA-ZNF131 and either FLAG-UHRF2 or Myc-hPc2. Cell extracts were immunoprecipitated with the HA antibody and immunoblotted with the indicated antibodies. Mr(K), relative molecular mass.