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. 2013 Feb 5;288(13):9145–9152. doi: 10.1074/jbc.M112.427880

FIGURE 2.

FIGURE 2.

A, matrix metalloprotease and TNF-α-converting enzyme (TACE) inhibitor GM6001 does not prevent LPS-induced sensitization of microglia to caspase inhibition. Cultures were preincubated for 1 h with 25 μm GM6001 or dimethyl sulfoxide for controls prior to addition of 100 ng/ml LPS for 24 h and subsequent addition of 50 μm Z-VAD for a further 24 h. Data represent mean values, and error bars represent S.E. of % dead microglia (n = 4). B, supernatants were collected prior to quantification of death in A, and TNF-α release was measured by ELISA. Data represent mean values, and error bars are S.E. (n = 4). C, cultures were treated with LPS or recombinant TNF-α (50 ng/ml) in the presence of either 2 μg/ml normal IgG or 2 μg/ml TNF-α blocking antibody for 24 h prior to addition of Z-VAD and quantification of cell death a further 24 h later. Data represent mean values, and error bars represent S.E. of % dead microglia (n = 3). * = p < 0.05, ** = p < 0.01, *** = p < 0.001. NS, not significant.