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. 2013 Feb 5;288(13):9145–9152. doi: 10.1074/jbc.M112.427880

FIGURE 3.

FIGURE 3.

Caspase inhibitor-induced death of inflamed microglia is inhibited by RIPK1 inhibitor necrostatin-1. A–D, cultures were treated with 100 ng/ml LPS (A and B) or 50 ng/ml TNF-α (C and D) for 24 h prior to 1 h treatment with 30 μm necrostatin-1 followed by addition of Z-VAD (A and C) or IETD (B and D) for a further 24 h and quantification of cell death. Data represent mean values, and error bars are S.E. of % dead microglia (n = 3). E, cultures were treated with 100 ng/ml LPS for 24 h prior to 1 h treatment with 30 μm necrostatin-1 or necrostatin-1-I (an inactive analog of necrostatin-1) followed by addition of Z-VAD (50 μm) for a further 24 h and quantification of cell death (n = 3). NS, not significant. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.