MB increases OCR and decreases ECAR in U87 cells.
A, OCR of human female primary astrocytes and U87 glioma cells is shown. Although U87 cells have a higher base-line OCR, both were able to obtain a similar maximum OCR upon FCCP treatment. B, ECAR of female primary astrocytes and U87 glioma cells is shown. U87 cells maintained a greater ECAR compared with human female astrocytes. C, effects of MB at concentrations of 100 nm, 1 μm, and 10 μm on U87 cell OCR are shown. D, effects of MB at concentrations of 100 nm, 1 μm, and 10 μm on U87 cell ECAR are shown. E, MB significantly increased U87 cell OCR at concentrations of 1 and 10 μm. F, MB significantly decreased U87 cell ECARs at concentrations of 1 and 10 μm. * indicates p < 0.05 by 1-way ANOVA and Tukey's post-hoc test.