Skip to main content
. 2013 Feb 19;288(13):9261–9271. doi: 10.1074/jbc.M112.433730

FIGURE 4.

FIGURE 4.

Enforced expression of miR-145 and miR-143 retarded odontoblast differentiation. A, lentivirus mediated miR-145 and miR-143 overexpression in mDPC6T. Shown are mRNA (B) and protein levels (C) of typical odontoblast markers after miR-145 and miR-143 overexpression. mRNA (D) and protein levels (E) of typical odontoblast markers 24 h after LNA-miR-145 and LNA-miR-143 inhibitor transfection are shown. F, validation of efficiency of lentivirus mediated miR-145 sponge and miR-143 sponge using 293FT cell line. G, Von-Kossa staining showing miR-145 and miR-143 overexpression inhibits mineralization of odontoblast. H, Von-Kossa staining showing down-regulation of miR-145 and miR-143 could accelerate or direct mineralization of odontoblast. Photos were taken at 200× magnification. Asterisks indicate statistical significance compared with the negative control groups. *, p < 0.05; **, p < 0.01.