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. 2013 Feb 19;288(13):9261–9271. doi: 10.1074/jbc.M112.433730

FIGURE 6.

FIGURE 6.

miR-145 directly targeted Klf4 and Osx in odontoblasts. A, mRNA of Klf4 and Osx when miR-145 and miR-143 were up-regulated and down-regulated. B, protein levels of KLF4 and OSX when miR-145 and miR-143 were up-regulated. C, protein levels of KLF4 and OSX when miR-145 and miR-143 were down-regulated. D, in silico assay reveals binding sites for miR-145 with 3′-UTR of Klf4 and Osx. E, validation of miR-145 binding sites using Dual-Luciferase assay, miR-145 can depress the luciferase activity of pMIR-reporter with the 3′-UTR from Klf4 and Osx compared with their mutant 3′-UTRs. All data are presented as mean ± S.D. and are based on triplicate independent experiments. Asterisks indicate statistical significance compared with the negative control groups: *, p < 0.05; **, p < 0.01. mmu, M. musculus.