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. 2013 Feb 6;288(13):9363–9372. doi: 10.1074/jbc.M113.453712

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Monomeric Vac14 mutants do not interact with Fab1 or Fig4. vac14Δ FAB1-Myc cells were transformed with plasmids encoding FLAG-tagged Vac14, Vac14^SS, or Vac14^CRY mutants. FLAG-Vac14 proteins were then immunoprecipitated with monoclonal anti-FLAG antibodies and subjected to SDS-PAGE and immunoblotting. A, co-IP of Fab1-Myc with FLAG-tagged wild-type Vac14 but not with corresponding mutants. B, co-IP of Fig4 with FLAG-tagged wild-type Vac14 but not with corresponding Vac14 mutants. vac14Δ FAB1-Myc cells carrying an empty vector (−) were used as a negative control. Input lanes represent 20% of total protein used in IP. * (asterisk) indicates nonspecific.