FIGURE 6.

Monomeric Vac14 mutants display vacuolar defects. A and B, vac14Δ cells transformed with either empty vector or vectors expressing Vac14, Vac14^SS, or Vac14^NG were grown to log phase. Vacuoles were then labeled with FM4-64 as described under “Experimental Procedures.” A, vacuolar morphology of log-phase cells. B, vacuolar morphology of cells exposed to a 10-min hyperosmotic shock with 0.9 m NaCl. The respective FM4-64 (left) and differential interference contrast (DIC, right) are shown for each condition. The scale bar represents 10 μm.