FIGURE 4.

Binding and dissociation kinetics for F₁(−δϵ) and sensor-bound biotinylated ϵ, with or without ϵCTD. Biotinylated Bap-ϵ (wild type or ϵ88stop, as noted) was loaded onto streptavidin biosensors. At time 0, each sensor was transferred from buffer alone to a sample containing 15 nm F₁(−δϵ). After 900 s (vertical dashed line), each sensor was moved into buffer without F₁(−δϵ). Black lines, experimental data; red lines, the kinetic fits for each ϵ, from global regression of kinetic data at varied concentrations of F₁(−δϵ). Kinetic parameters and K_D values derived from the fittings are summarized in Table 2.