TABLE 1.

Inhibition of F₁(−δϵ) by variants of subunit ϵ

Results of nonlinear regression for data shown in Fig. 2 and a data set with untagged WT ϵ. Equation 1 was used (see “Experimental Procedures”).

ϵ	NTP/Mg2+ ratio	Activity of F1(−δϵ) (A0)a	Activity of ϵ-saturated F1 (Ae/A0)b ± S.E.c	KI ± S.E.c
	mm	μmol min−1 mg−1	%	nm
Wild type	ATP 2:1	78	7.9 ± 0.6	0.49 ± 0.02
Wild-type H6-ϵ	ATP 2:1	78	6.8 ± 0.8	0.67 ± 0.04
	ATP 1:2	37	7.9 ± 0.5	0.46 ± 0.02
	GTP 1:2	94	7.3 ± 0.7	0.87 ± 0.04
H6-ϵ88stop	ATP 2:1	73	76.3 ± 1.2	12.4 ± 1.9
	ATP 1:2	36	54.6 ± 1.4	7.2 ± 0.8
	GTP 1:2	95	84 ± 2.0	13 ± 7
H6-ϵ101C/121C, + 1 mm DTT	ATP 2:1	80	31 ± 1	1.2 ± 0.1
	ATP 1:2	41	27 ± 1	0.98 ± 0.06
H6-ϵ101C/121C, disulfide-bonded	ATP 2:1	80	69 ± 2	23 ± 3.7
	ATP 1:2	38	58 ± 1	31 ± 2

^a Hydrolysis activity units are μmol·min⁻¹·mg⁻¹ F₁(−δϵ).

^b Activity of ϵ-saturated F₁(−δϵ), A_e, is listed as a percentage of A₀, the measured activity of F₁(−δϵ) alone.

^c S.E., standard error from nonlinear regression.