FIGURE 6.
4-AP inhibits BLINaC in a voltage-dependent manner. a, representative macroscopic current traces of rat BLINaC (A443T) in a voltage-clamped CHO cell before (left) and after (right) 10 mm 4-AP. Current was evoked with a 10-mV step protocol ranging from 100 to −100 mV from a holding potential of 0 mV. Symmetrical NaCl solutions were used in these experiments. b, summary graph of the macroscopic current-voltage (I-V) relation for recombinant rat BLINaC expressed in CHO cells in the absence (black) and presence (gray; 10 mm) of 4-AP. Data are from experiments identical to that in a (n = 5). c, corresponding conductance-voltage (G-V) curve for rat BLINaC in CHO cells in the presence of 10 mm 4-AP. Normalized conductance was fit with a Boltzmann distribution. d, summary graph showing the voltage dependence of the Kd for 10 mm 4-AP on BLINaC. The normalized (I4-AP/Icon) I-V relation for BLINaC used to calculate Kd is shown in the inset. Data from experiments are identical to those in a.