FIGURE 8.

4-AP decreases the unitary current of BLINaC in a dose-dependent manner. Representative segments from a typical (n = 4) continuous single-channel current trace of recombinant BLINaC (A443T) expressed in CHO cells in an excised, outside-out patch clamped to −60 mV before and after 4-AP in the absence and presence of amiloride. This patch contains at least two BLINaC channels. The pipette and bath contained symmetrical LiCl solutions. Inward current is down, and closed and open 1 and open 2 states are indicated with dashed lines and C, O₁, and O₂, respectively. The point at which amiloride was added is indicated by a solid gray arrowhead.