FIGURE 4.

Knockdown of PKCι expression with siRNA blocked Wnt3a-dependent neurite outgrowth. A, representative confocal micrographs, PKCι siRNA versus luciferase siRNA control. TC-32 cells were treated with the indicated siRNA reagents and 48 h later incubated for 3 h with serum-free culture medium ± Wnt3a (100 ng/ml). B, quantitative summary of the percentage of cells with a neurite at least one cell diameter in length, normalized to luciferase siRNA control without Wnt3a treatment (typically ∼10% cells have a long neurite in this control). The bar graph shows the results of three independent experiments (mean ± S.D.). C, immunoblot confirmed knockdown of endogenous PKCι protein. Ctl., control; IB, immunoblot; Luc, luciferase.