Co-immunoprecipitation of endogenous PKCι and Dvl2 following Wnt3a treatment. TC-32 cells were incubated ± Wnt3a for 3 h, and cell lysates were prepared for immunoprecipitation with Dvl2 rabbit polyclonal antiserum. Pelleted protein and cell lysates were immunoblotted as indicated. An arrow points to immunoprecipitated PKCι. *, IgG heavy chain. **, note increased relative intensity of slower migrating Dvl2 band. IB, immunoblot; IP, immunoprecipitation.