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Co-immunoprecipitation of FLAG-tagged Dvl2 derivatives and endogenous PKCι. FLAG-tagged mouse Dvl2 WT or a Wnt-induced phosphorylation mutant containing four alanine substitutions (Dvl2 P4m) was stably expressed in TC-32 using a lentiviral vector. The cells were lysed, and the proteins were precipitated with FLAG antibody. Pellets and cell lysates were immunoblotted as indicated. An arrow points to immunoprecipitated PKCι. *, IgG heavy chain. IB, immunoblot; IP, immunoprecipitation.
