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. 2013 Feb 7;288(13):9457–9467. doi: 10.1074/jbc.M112.415703

FIGURE 2.

FIGURE 2.

EMSA with recombinant His-MEF/ELF4, His, GST, and GST-Wt-NPM1. His-MEF/ELF4 was incubated with GST and GST-Wt-NPM1 at room temperature prior to EMSA by using a biotin-conjugated APET probe (lanes 1–4). An excess amount of unlabeled APET competitor was added to the reaction mixtures (lanes 5 and 6).