FIGURE 5.

Immunohistochemical staining of PV+ve GABAergic interneurons in control (15 years old) and two different LD brain biopsy samples (LD1, 17 years and LD2, 23 years old). Arrow indicates PV+ve neurons. Scale bar, 20 μm. For counting PV-immunostained cells in cortex area, two serial sections in each sample (two controls and two LD) were used. At least three 20× images of same size (500 μm × 400 μm) in each section were used for counting PV-immunostained cells. The numbers of PV+ve neurons in control brain was 9.7 ± 2.68, whereas in LD brain it was 1.8 ± 0.53 (2.2 in LD1 and 1.45 in LD2). LD1 showed approximately 78% reduction of PV-stained cells whereas LD2 showed ∼85% reduction compared with control.