TABLE 2.

Effect of nucleotide and RLC phosphorylation on binding of myosin-18Aβ to actin

Data show the extent of binding of myosin-18Aβ-S1 to 10 μm actin for cases where the myosin was first phosphorylated with MLCK or where the binding was conducted in the presence of ADP or ATP or in the absence of nucleotide (APO). The amount of binding predicted by the simulation is marked as “Simulation” (see below).

Assay	Fraction of myosin-18Aβ-S1 bound
Apo	0.048 ± 0.011
ATP	0.032 ± 0.006
ADP	0.061 ± 0.027
MLCK	0.074 ± 0.0002
Simulation	0.074 ± 0.0431