FIGURE 5.

Y2H analysis of the interaction of μ3A with cytosolic tails containing a YXXØ motif. A–C, yeast were co-transformed with plasmids encoding Gal4bd fused to the wild-type or Tyr-to-Ala mutant of the cytosolic tails of TGN38, CD63, or Lamp-1 constructs indicated on the left, and Gal4ad fused to wild-type or mutant μ3A constructs indicated on top of each panel. B, Y2H analysis of μ3A with mutations on the YXXØ-binding site. C, Y2H analysis of μ3A with mutations on a putative YX(FYL)(FL)E-binding site. Mouse p53 fused to Gal4bd and SV40 large T antigen (T Ag) fused to Gal4ad were used as controls. Co-transformed cells were spotted onto His-deficient (−His) or His-containing (+His) plates and incubated at 30 °C. Growth is indicative of interactions.