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. 2013 Feb 14;49(3):162–169. doi: 10.1007/s11626-013-9588-2

Figure 4.

Figure 4.

MP1 enhances cell cycle progression. AGS-GR cells were grown and treated with siCtr, siMP1, or siCycD1. Cells were treated with 10 nM gastrin without medium replacement 24 h after transfection and then grown for another 24 h. Finally, the cells were harvested in citrate buffer and treated with buffers A, B, and C according to a slightly modified version of the Vindelov protocol (Vindelov et al. 1983). The proportion of cells in S-phase of the cell cycle in untreated (black) and gastrin-treated (red) bars. DNA histograms were created from 20,000 propidium iodide stained nuclei. Representative histograms are shown, and data is presented as percent cells in S-phase mean ± SD (n = 3). Using qRT-PCR, MP1 depletion was found to be approximately 90% (data not shown).