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Increased expression of hedgehog ligand and hedgehog target genes in fatty livers. RNA was isolated from the livers of chow-fed controls (CON, n = 4), HF mice (n = 4), and HF + EtOH mice (n = 9) and analyzed by QRT-PCR. Results are graphed as mean ± SD for Sonic hedgehog (Shh) (A), Patched (Ptc) (B), Gli2 (C), and Frizzled related peptide (Frp) 1 (D). *P < .05; **P < .005.
